high five insect cells Search Results


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Becton Dickinson sf-9 or high five insect cells
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Lonza cell line bti-tn5b1-4
<t>Tn5</t> insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.
Cell Line Bti Tn5b1 4, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SAFC Biosciences Inc insect sf9 cells
<t>Tn5</t> insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.
Insect Sf9 Cells, supplied by SAFC Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fisher Scientific high five insect cell
<t>Tn5</t> insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.
High Five Insect Cell, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioExpress high 5 insect cells
<t>Tn5</t> insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.
High 5 Insect Cells, supplied by BioExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza high-five insect cells (trichoplusia ni, female, ovarian)
<t>Tn5</t> insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.
High Five Insect Cells (Trichoplusia Ni, Female, Ovarian), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Glaxo Smith high five (trichoplusia ni) insect cells
<t>Tn5</t> insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.
High Five (Trichoplusia Ni) Insect Cells, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pfizer Inc p-gp- and abcg2-expressing high-five insect cell crude membranes
<t>Tn5</t> insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.
P Gp And Abcg2 Expressing High Five Insect Cell Crude Membranes, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Glaxo Smith high five insect cells
<t>Tn5</t> insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.
High Five Insect Cells, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Tn5 insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.

Journal: Toxins

Article Title: Anti-Virulence Strategy against the Honey Bee Pathogenic Bacterium Paenibacillus larvae via Small Molecule Inhibitors of the Bacterial Toxin Plx2A

doi: 10.3390/toxins13090607

Figure Lengend Snippet: Tn5 insect cell culture assay with Plx2A and inhibitors. The cells were incubated with Plx2A and inhibitors for two days, and afterwards DyLight 488 phalloidin- and DAPI-stained to visualize the F-actin cytoskeleton and the cell nuclei, respectively. Bi-nucleated cells are indicative of Plx2A activity. Mono-nucleated cells comparable to the controls are indicative of inhibitor activity as was the case for M3 at 300 µM and Acacetin at 30 µM.

Article Snippet: The cell line BTI-Tn5B1-4 (Tn5) derived from the cabbage looper Trichoplusia ni (Lepidotera) was cultured at 27 °C in SF 900 II medium (Lonza, Basel, Switzerland) until confluency.

Techniques: Cell Culture, Incubation, Staining, Activity Assay

Quantification of bi-nucleated cells in the Tn5 insect cell culture assay. Per treatment group, around 100 cells were analyzed for the number of nuclei. The percentage of bi-nucleated cells in the treatment group that received only Plx2A without any inhibitors was set to 100% and the reduction in the groups that received Plx2A with inhibitors was then determined. Bars depict the percentage of bi-nucleated cells relative to the treatment with Plx2A alone without any inhibitor. The data was analyzed with pairwise chi-square tests with Bonferroni correction comparing all treatment groups to the treatment with Plx2A without inhibitor to check for a reduction in the number of bi-nucleated cells.

Journal: Toxins

Article Title: Anti-Virulence Strategy against the Honey Bee Pathogenic Bacterium Paenibacillus larvae via Small Molecule Inhibitors of the Bacterial Toxin Plx2A

doi: 10.3390/toxins13090607

Figure Lengend Snippet: Quantification of bi-nucleated cells in the Tn5 insect cell culture assay. Per treatment group, around 100 cells were analyzed for the number of nuclei. The percentage of bi-nucleated cells in the treatment group that received only Plx2A without any inhibitors was set to 100% and the reduction in the groups that received Plx2A with inhibitors was then determined. Bars depict the percentage of bi-nucleated cells relative to the treatment with Plx2A alone without any inhibitor. The data was analyzed with pairwise chi-square tests with Bonferroni correction comparing all treatment groups to the treatment with Plx2A without inhibitor to check for a reduction in the number of bi-nucleated cells.

Article Snippet: The cell line BTI-Tn5B1-4 (Tn5) derived from the cabbage looper Trichoplusia ni (Lepidotera) was cultured at 27 °C in SF 900 II medium (Lonza, Basel, Switzerland) until confluency.

Techniques: Cell Culture